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Fresh Whole Blood- ACT+, ACT-LR,
PT, APTT Cuvettes |
| 1. |
Place cuvette into analyser. Analyser with verify the test
to be run and pre-warm the cuvette to 37°C. |
| 2. |
Collect a minimum of 0.2 ml of fresh whole blood with a syringe
or by fingerstick (PT cuvettes only). |
| 3. |
Immediately dispense 1 drop of blood into the sample well of
the test cuvette- filling the well from the bottom of the well
up- this will avoid addition of air bubbles into the blood sample. |
| 4. |
A sufficient quantity of blood must be added directly to the
center sample well to fill it flush to the top. |
| 5. |
Should a large drop of blood extend above the center sample
well on the cuvette, push any excess blood over into the outer
sample well on the cuvette. |
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|
| |
Citrated Whole Blood- Citrated
PT and Citrated APTT Cuvettes |
| 1. |
Collect blood in an evacuated tube containing sodium citrate
(3.2% or 3.8%). Mix gently. |
| 2. |
Before testing, invert the test tube at least four times to
ensure complete mixing of sample. |
| 3. |
Dispense 1 drop of citrated blood into the sample well of the
test cuvette- filling the well from the bottom of the well up-
this will avoid addition of air bubbles into the blood sample. |
| 4. |
A sufficient quantity of blood must be added directly to the
center sample well to fill it flush to the top. |
| 5. |
Should a large drop of blood extend above the center sample
well on the cuvette, push any excess blood over into the outer
sample well on the cuvette. |
| |
|
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NOTES: |
| 1. |
A minimum volume of 50 ul blood sample is required for the
HEMOCHRON Signature cuvette system. |
| 2. |
The Signature instrument displays “Sample too large” or “Sample
too small” if an excessive or inadequate blood sample volume
has been provided. |
